diff --git a/stubs/pyinstaller/@tests/stubtest_allowlist.txt b/stubs/pyinstaller/@tests/stubtest_allowlist.txt
index 626c29c3ef15..f04987c54a02 100644
--- a/stubs/pyinstaller/@tests/stubtest_allowlist.txt
+++ b/stubs/pyinstaller/@tests/stubtest_allowlist.txt
@@ -43,7 +43,6 @@ PyInstaller.utils.hooks.django
 PyInstaller.utils.hooks.gi
 PyInstaller.utils.hooks.qt
 PyInstaller.utils.hooks.setuptools
-PyInstaller.utils.hooks.tcl_tk
 PyInstaller.utils.misc
 PyInstaller.utils.osx
 PyInstaller.utils.run_tests
diff --git a/stubs/pyinstaller/PyInstaller/building/splash.pyi b/stubs/pyinstaller/PyInstaller/building/splash.pyi
index 506def4b1888..d7723bc76f53 100644
--- a/stubs/pyinstaller/PyInstaller/building/splash.pyi
+++ b/stubs/pyinstaller/PyInstaller/building/splash.pyi
@@ -1,6 +1,7 @@
 from _typeshed import StrPath
 
 from PyInstaller.building.datastruct import Target, _TOCTuple
+from PyInstaller.utils.hooks.tcl_tk import TclTkInfo
 
 # Referenced in https://pyinstaller.org/en/stable/spec-files.html#example-merge-spec-file
 # Not to be imported during runtime, but is the type reference for spec files which are executed as python code
@@ -42,4 +43,7 @@ class Splash(Target):
         always_on_top: bool = True,
     ) -> None: ...
     def assemble(self) -> None: ...
+    # This private method is the only way to match Splash Screen support validation without triggering an actual build
+    @staticmethod
+    def _check_tcl_tk_compatibility(tcltk_info: TclTkInfo) -> None: ...
     def generate_script(self) -> str: ...
diff --git a/stubs/pyinstaller/PyInstaller/utils/hooks/tcl_tk.pyi b/stubs/pyinstaller/PyInstaller/utils/hooks/tcl_tk.pyi
new file mode 100644
index 000000000000..1a3f196f8f02
--- /dev/null
+++ b/stubs/pyinstaller/PyInstaller/utils/hooks/tcl_tk.pyi
@@ -0,0 +1,20 @@
+from typing import Final
+
+class TclTkInfo:
+    TCL_ROOTNAME: Final = "_tcl_data"
+    TK_ROOTNAME: Final = "_tk_data"
+    def __init__(self) -> None: ...
+    available: bool
+    tkinter_extension_file: str | None
+    tcl_version: tuple[int, int] | None
+    tk_version: tuple[int, int] | None
+    tcl_threaded: bool
+    tcl_data_dir: str | None
+    tk_data_dir: str | None
+    tcl_module_dir: str | None
+    is_macos_system_framework: bool
+    tcl_shared_library: str | None
+    tk_shared_library: str | None
+    data_files: list[tuple[str, str, str]]
+
+tcltk_info: TclTkInfo