diff --git a/change/@fluentui-web-components-0c22cad8-1535-4970-b928-3554886f412f.json b/change/@fluentui-web-components-0c22cad8-1535-4970-b928-3554886f412f.json
new file mode 100644
index 00000000000000..11a7357fc67675
--- /dev/null
+++ b/change/@fluentui-web-components-0c22cad8-1535-4970-b928-3554886f412f.json
@@ -0,0 +1,6 @@
+{
+  "type": "patch",
+  "comment": "fix: restore menu list keyboard navigation after reconnect",
+  "packageName": "@fluentui/web-components",
+  "email": "aseebshibin4@gmail.com"
+}
diff --git a/packages/web-components/src/menu-list/menu-list.spec.ts b/packages/web-components/src/menu-list/menu-list.spec.ts
index 27206f1f1e4ab1..4ede893f8633a9 100644
--- a/packages/web-components/src/menu-list/menu-list.spec.ts
+++ b/packages/web-components/src/menu-list/menu-list.spec.ts
@@ -78,6 +78,36 @@ test.describe('MenuList', () => {
     await expect(firstItem).toBeFocused();
   });
 
+  test('should preserve arrow key navigation after being moved in the DOM', async ({ fastPage }) => {
+    const { element } = fastPage;
+    const menuItems = element.locator(MenuItemTagName);
+
+    await fastPage.setTemplate();
+
+    // Move the menu list to trigger its disconnect/reconnect lifecycle.
+    await element.evaluate(node => {
+      const parent = node.parentElement;
+
+      if (!parent) {
+        throw new Error('Menu list must have a parent element');
+      }
+
+      const nextSibling = node.nextSibling;
+      node.remove();
+      parent.insertBefore(node, nextSibling);
+    });
+
+    await element.evaluate(node => {
+      node.focus();
+    });
+
+    await expect(menuItems.first()).toBeFocused();
+
+    await element.press('ArrowDown');
+
+    await expect(menuItems.nth(1)).toBeFocused();
+  });
+
   test('should focus before the queued item refresh completes', async ({ fastPage, page }) => {
     await fastPage.setTemplate('');
     await page.evaluate(
diff --git a/packages/web-components/src/menu-list/menu-list.ts b/packages/web-components/src/menu-list/menu-list.ts
index 76dae478d0da80..234850d94d3805 100644
--- a/packages/web-components/src/menu-list/menu-list.ts
+++ b/packages/web-components/src/menu-list/menu-list.ts
@@ -20,6 +20,7 @@ export class MenuList extends BaseMenuList {
 
   disconnectedCallback() {
     this.fg?.disconnect();
+    this.fg = undefined;
     super.disconnectedCallback();
   }