Run these from inside this directory — the paths in each script are relative to it.
cd examples
./splitfastqs_example.sh
./refsplice_example.sh
./count_example.sh
./call_example.sh
splitfastqs_example.sh splits the bundled sample FASTQ into one file per barcode. The
other three scripts do not consume its output. The aligned perturbed BAMs are already
checked in under example_data/TX46_Prosalpha3_bams/, so the analysis runs without
repeating the alignment step. All three share --results-dir=results and the run name
TX46_Prosalpha3, and write into results/.
Two notes:
count_example.shwrites amutations/directory insideexample_data/TX46_Prosalpha3_bams/, which is part of the tree. Delete it after a run.- The final
results/TX46_Prosalpha3_mutation_statuses.txtshould match the checked-inexample_data/ref_TX46_Prosalpha3_mutation_statuses.txt. The reference file's rows are in a different order, so compare withsorton both sides.
For a walkthrough of what each stage does and how to adapt this to your own data, see ../docs/tutorial.md.